New Rp - Hplc Method Development and Validation for Analysis of Almotriptan
نویسنده
چکیده
A simple, rapid and precise reverse phase high performance liquid chromatography method was developed for the analysis of Almotriptan. Chromatographic separation of Almotriptan was performed by using a kromosil C18 column (250 x 4.6mm, 5 μm) as stationary phase with a mobile phase comprising of 1% Triethyl amine : acetonitrile:methanol: 05:55:40 (v/v) at a flow rate of 1.0ml/min and UV detection at 230nm. The linearity of Almotriptan is in the range of 10 ppm to 60 ppm. The limit of detection for Almotriptan was found to be 0.05ppm. The proposed method was found to be accurate, precise and rapid for the analysis of Almotriptan.
منابع مشابه
A New Stability Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Dolutegravir and Rilpivirine in Bulk and its Dosage Forms
A New Stability Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Dolutegravir and Rilpivirine in Bulk and its Dosage Forms. Abstract The objective of the work is to develop and validate a new, simple, highly sensitive, stability indicating RP-HPLC method for simultaneous estimation of Dolutegravir and Rilpivirine in bulk and its dosage f...
متن کاملDevelopment and validation of a fast, simple and specific stability indicating RP-HPLC method for determination of Dexpanthenol in eye gel formulation.
AIn this study a simple and reliable stability-indicating RP-HPLC method was developed and validated for analysis of Dexpanthenol in an artificial tear formulation. The chromatographic separation was performed on a HPLC C18 column (25.0 cm 4.6 mm, 5 m) using a mixture of 0.037 M monobasic potassium phosphate in water (adjusted with 0.1% (v/v) phosphoric acid to a pH of 3.2) and methanol (90:...
متن کاملDevelopment and validation of a fast, simple and specific stability indicating RP-HPLC method for determination of Dexpanthenol in eye gel formulation.
AIn this study a simple and reliable stability-indicating RP-HPLC method was developed and validated for analysis of Dexpanthenol in an artificial tear formulation. The chromatographic separation was performed on a HPLC C18 column (25.0 cm 4.6 mm, 5 m) using a mixture of 0.037 M monobasic potassium phosphate in water (adjusted with 0.1% (v/v) phosphoric acid to a pH of 3.2) and methanol (90:...
متن کاملSimultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form
The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...
متن کاملRP-HPLC method development and validation for determination of eptifibatide acetate in bulk drug substance and pharmaceutical dosage forms
A new, rapid, economical and isocratic reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of eptifibatide acetate, a small synthetic antiplatelet peptide, in bulk drug substance and pharmaceutical dosage forms. The developed method was validated as per of ICH guidelines. The chromatographic separation was achieved isocratically on C18 colum...
متن کامل